cpg 1826 Search Results


r848  (MedChemExpress)
93
MedChemExpress r848
LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist <t>R848</t> (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001
R848, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/r848/product/MedChemExpress
Average 93 stars, based on 1 article reviews
r848 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
cpg  (MedChemExpress)
93
MedChemExpress cpg
LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist <t>R848</t> (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001
Cpg, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cpg/product/MedChemExpress
Average 93 stars, based on 1 article reviews
cpg - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
cpg odn 1826  (TriLink)
90
TriLink cpg odn 1826
LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist <t>R848</t> (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001
Cpg Odn 1826, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cpg odn 1826/product/TriLink
Average 90 stars, based on 1 article reviews
cpg odn 1826 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
cpg odn 1668  (GeneWorks)
90
GeneWorks cpg odn 1668
LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist <t>R848</t> (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001
Cpg Odn 1668, supplied by GeneWorks, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cpg odn 1668/product/GeneWorks
Average 90 stars, based on 1 article reviews
cpg odn 1668 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
oligodeoxynucleotide cpg odn1826  (TIB MOLBIOL)
90
TIB MOLBIOL oligodeoxynucleotide cpg odn1826
LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist <t>R848</t> (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001
Oligodeoxynucleotide Cpg Odn1826, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligodeoxynucleotide cpg odn1826/product/TIB MOLBIOL
Average 90 stars, based on 1 article reviews
oligodeoxynucleotide cpg odn1826 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
cpg oligodeoxynucleotides with a phosphorthioate backbone cpg-odn 1826  (Microsynth ag)
90
Microsynth ag cpg oligodeoxynucleotides with a phosphorthioate backbone cpg-odn 1826
LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist <t>R848</t> (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001
Cpg Oligodeoxynucleotides With A Phosphorthioate Backbone Cpg Odn 1826, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cpg oligodeoxynucleotides with a phosphorthioate backbone cpg-odn 1826/product/Microsynth ag
Average 90 stars, based on 1 article reviews
cpg oligodeoxynucleotides with a phosphorthioate backbone cpg-odn 1826 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
cy5-labeled cpg-b 1826  (Nihon Gene Research Laboratories)
90
Nihon Gene Research Laboratories cy5-labeled cpg-b 1826
LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist <t>R848</t> (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001
Cy5 Labeled Cpg B 1826, supplied by Nihon Gene Research Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cy5-labeled cpg-b 1826/product/Nihon Gene Research Laboratories
Average 90 stars, based on 1 article reviews
cy5-labeled cpg-b 1826 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
cpg 1826  (Coley Pharmaceutical)
90
Coley Pharmaceutical cpg 1826
LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist <t>R848</t> (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001
Cpg 1826, supplied by Coley Pharmaceutical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cpg 1826/product/Coley Pharmaceutical
Average 90 stars, based on 1 article reviews
cpg 1826 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
cpg-odn c-class odn 2395  (Coley Pharmaceutical)
90
Coley Pharmaceutical cpg-odn c-class odn 2395
LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist <t>R848</t> (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001
Cpg Odn C Class Odn 2395, supplied by Coley Pharmaceutical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cpg-odn c-class odn 2395/product/Coley Pharmaceutical
Average 90 stars, based on 1 article reviews
cpg-odn c-class odn 2395 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
immunostimulatory odn cpg 1826  (Oligos Etc)
90
Oligos Etc immunostimulatory odn cpg 1826
LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist <t>R848</t> (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001
Immunostimulatory Odn Cpg 1826, supplied by Oligos Etc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immunostimulatory odn cpg 1826/product/Oligos Etc
Average 90 stars, based on 1 article reviews
immunostimulatory odn cpg 1826 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
cpg 1826  (Eurofins)
90
Eurofins cpg 1826
LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist <t>R848</t> (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001
Cpg 1826, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cpg 1826/product/Eurofins
Average 90 stars, based on 1 article reviews
cpg 1826 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
cpg7909 adjuvant  (TriLink)
90
TriLink cpg7909 adjuvant
LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist <t>R848</t> (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001
Cpg7909 Adjuvant, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cpg7909 adjuvant/product/TriLink
Average 90 stars, based on 1 article reviews
cpg7909 adjuvant - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist R848 (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001

Journal: Cell Communication and Signaling : CCS

Article Title: Induction of LY6E regulates interleukin-1β production, potentially contributing to the immunopathogenesis of systemic lupus erythematosus

doi: 10.1186/s12964-025-02140-z

Figure Lengend Snippet: LY6E deficiency inhibited IL-1β expression induced by stimulation with IFN-α and ICs. BMDMs (2 × 10. 6 ) were electroporated with 300 nM LY6E siRNA (siLY6E) or control siRNA (siCtl) and then stimulated with or without various stimuli as indicated for 24 h. The concentrations of the individual stimuli used were as follows: the TLR7/8 agonist R848 (2.5 μg/ml), the TLR3 agonist PolyIC (10 μg/ml), LPS (100 ng/ml), IFN-α (100 U/ml), and ICs (10 μg/ml). The mRNA and protein levels of LY6E were determined by qPCR ( A ) and Western blotting ( B ), respectively. The mRNA expression of several inflammation-associated molecules induced by various stimuli with or without LY6E deficiency conditions was determined ( C ). The IFN-α- and IC-induced expression of IL-1β with or without LY6E knockdown was determined by Western blotting ( D and E ). Each data point represents one mouse, and the values are fold changes relative to the mean of the siCtl in RT‒qPCR and Western blotting. For Western blotting, the samples were derived from the same experiment, and both the gels and the blots were processed in parallel. Statistical analysis was performed with Student’s t test to compare the means between two groups (A and B) or two-way ANOVA with Holm‒Sidak’s multiple comparisons test to compare differences among different treatments ( D and E ). * P < 0.05, ** P < 0.01, **** P < 0.0001

Article Snippet: Lipopolysaccharide (LPS) (tlrl-3pelp), Pam3CSK4 (tlrl-pms), PolyIC (tlrl-picw), R848 (tlrl-r848), CpG ODN1826 (tlrl-1826), H151 (inh-h151), Ac-YVAD-cmk (HY-16990) and voltage-dependent anion channel oligomerization inhibitor (VBIT-4, HY129122) were purchased from MedChemExpress LLC (Monmouth Junction, NJ, USA).

Techniques: Expressing, Control, Western Blot, Knockdown, Derivative Assay